Kinetic analysis of ethanol production by an acetate-resistant strain of recombinant Zymomonas mobilis |
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Authors: | Young Jae Jeon Charles J. Svenson Eva L. Joachimsthal Peter L. Rogers |
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Affiliation: | (1) Department of Biotechnology, University of New South Wales, Sydney, NSW 2052, Australia;(2) 2 9313 6710 |
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Abstract: | Zymomonas mobilis ZM4/AcR (pZB5), a mutant recombinant strain with increased acetate resistance, has been isolated following electroporation of Z. mobilis ZM4/AcR. This mutant strain showed enhanced kinetic characteristics in the presence of 12 g sodium acetate l–1 at pH 5 in batch culture on 40 g glucose, 40 g xylose l–1 medium when compared to ZM4 (pZB5). In continuous culture, there was evidence of increased maintenance energy requirements/uncoupling of metabolism for ZM4/AcR (pZB5) in the presence of sodium acetate; a result confirmed by analysis of the effect of acetate on other strains of Z. mobilis.NomenclaturemCell maintenance energy coefficient (g g–1 h–1)Maximum overall specific growth rate (1 h–1)Maximum specific ethanol production rate (g g–1 h–1)Maximum specific total sugar utilization rate (g g–1 h–1)Biomass yield per mole of ATP (g mole–1Ethanol yield on total sugars (g g–1)Biomass yield on total sugars (g g–1)True biomass yield on total sugars (g g–1) |
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Keywords: | acetate-tolerant mutant recombinant Zymomonas mobilis glucose/xylose fermentation lignocellulosic hydrolysates |
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