A method for measuring the content of a specific messenger ribonucleic acid in Escherichia coli |
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Authors: | J. O. Bishop and M. I. Irving |
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Affiliation: | Department of Genetics, University of Edinburgh, Edinburgh 9, U.K. |
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Abstract: | A method is described for measuring the porportion of a specific messenger RNA in the total RNA extracted from pulse-labelled cells. A model system consisting of total ribosomal RNA and Escherichia coli DNA is used to validate the method and to define the conditions under which it can be used. |
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