Assembly,Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging |
| |
| Authors: | Melissa Paulite Zahra Fakhraai Boris B. Akhremitchev Kerstin Mueller Gilbert C. Walker |
| |
| Affiliation: | Department of Chemistry, University of Toronto;Department of Chemistry, University of Wisconsin;Department of Chemistry, Duke University |
| |
| Abstract: | This paper aims to instruct the reader in the assembly and operation of an infrared near-field microscope for imaging beyond the diffraction limit. The apertureless near-field microscope is a light scattering-type instrument that provides infrared spectra at circa 20 nm resolution. A complete list of components and a step-by-step protocol for use is provided. Common errors in assembly and instrument tuning are discussed. A representative data set that shows the secondary structure of an amyloid fibril is presented. |
| |
| Keywords: | Cellular Biology Issue 33 nearfield imaging infrared amyloid fibril protein |
|
