Chemokine/cytokine profiling after rituximab: reciprocal expression of BCA-1/CXCL13 and BAFF in childhood OMS |
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Authors: | Pranzatelli Michael R Tate Elizabeth D Travelstead Anna L Verhulst Steven J |
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Institution: | a National Pediatric Myoclonus Center and Neuroimmunology Laboratory, Southern Illinois University School of Medicine, Springfield, IL 62794-9643, USA;b Department of Neurology, Division of Child and Adolescent Neurology, Southern Illinois University School of Medicine, Springfield, IL 62794-9643, USA;c Flow Cytometry Facility, Southern Illinois University School of Medicine, Springfield, IL 62794-9626, USA;d Department of Statistics and Research Consulting, Southern Illinois University School of Medicine, Springfield, IL 62794-9623, USA |
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Abstract: | The aim of the study was to test the hypothesis that B-cell repopulation following rituximab (anti-CD20) therapy is orchestrated by chemokines and non-chemokine cytokines. Twenty-five children with opsoclonus-myoclonus syndrome (OMS) received rituximab with or without conventional agents. A comprehensive panel of 40 chemokines and other cytokines were measured in serum by ELISA and multiplexed fluorescent bead-based immunoassay. Serum BAFF concentration changed dramatically (even after first infusion) and inversely with B-cell depletion/repopulation and CXCL13 concentration at 1, 3, and 6 months. Negative correlations were found for BAFF concentration vs blood B cell percentage and serum CXCL13 concentration; positive correlations with serum rituximab concentrations. Six months after initiation of therapy, no significant difference in the levels of APRIL, CXCL10, IL-6, or 17 other cytokines/chemokines were detected. These data reveal a major role for BAFF in peripheral B cell repopulation following rituximab-induced B-cell depletion, and novel changes in CXCL13. ClinicalTrials.gov NCT0024436. |
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Keywords: | ACTH Paraneoplastic syndrome B-cell repopulation B cell activating factor A proliferation-inducing ligand |
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