Improved sensitivity of genetic complementation of nitrate reductase deficient mutants via protoplast fusion |
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Authors: | I Saalbach R Grafe |
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Institution: | (1) Zentralinstitut für Genetik und Kulturpflanzenforschung der Akademie der Wissenschaften der DDR, 4325 Gatersleben, German Democratic Republic |
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Abstract: | An improved rapid assay for complementation testing of mutants of Nicotiana tabacum deficient in nitrate reductase is described. The test is based on measurement of in vivo nitrate reductase activity in 7 to 10 day old cultures derived from fusion-treated protoplast mixtures of the respective mutants as a criterion for complementation. It allows to detect complementing hybrids induced by the conventional droplet fusion technique in small numbers of protoplasts per assay (8×104).Abbreviations NR
nitrate reductase
- 2,4-D
dichlorophenoxy-acetic acid
- NAA
-naphthaleneacetic acid
- BAP
6-benzylaminopurine
- PEG
polyethylene glycol |
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Keywords: | |
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