心脏特异新基因Lrrc10的分子克隆与特性分析

采用表达序列标签(EST)介导的基因克隆和表达谱分析，从小鼠心脏克隆了一个心脏特异新基因Lrrc10（GenBank Acc No. AF527781）.该基因cDNA全长为1 410 bp，定位于小鼠染色体10D2，在基因组中无内含子.Lrrc10的最大开放阅读框编码的假想蛋白由274个氨基酸组成，含有7个亮氨酸重复基序.同源性检索未发现有整体同源性的已知基因.EST数据库中支持该基因cDNA序列的全部18条EST均来自小鼠心脏组织.对小鼠的不同组织cDNA的RT-PCR检测证实该基因主要在心脏中强表达，在肺低表达，而在其他组织中不表达或表达很弱.因此该基因是心脏特异的富亮氨酸重复超家族新成员.

Molecular Cloning, Characterization and Expression of Lrrc10:a Novel Mouse Heart-specific Member of Leucine-rich Repeat Superfamily

A novel mouse heart-specific gene, Lrrc10(GenBank Acc No. AF527781), was cloned from mouse embryo heart by application of EST assembly and RT-PCR. The cDNA of Lrrc10 was 1 410 bp and intronless. This gene was mapped to mouse chromosome 10D2 by BLAST search to mouse genome. The longest ORF of the cDNA encoded a putative proteins of 274 amino acids. Seven leucine rich repeat motifs were present between 53 amino acid to 212 amino acid. No known gene or protein was significantly homologous to Lrrc10 or its deduced protein. However, XM-137268, a predicted human gene submitted by NCBI genome annotation project,shared high identity to Lrrc10. It may be concluded that XM-137268 was the uncloned human orthologue of Lrrc10. BLAST to EST database showed that cDNA of Lrrc10 was supported by 18 ESTs, all of them were from mouse heart. RT-PCR performed on a panel of different mouse tissues demonstrated that expression of Lrrc10 was strongly in heart, low in lung, not or very weakly in other tissues. These results suggest that Lrrc10 is a novel heart specific member of leucine rich repeat superfamily. As far as, no heart-specific member of this family was reported before.