Trehalose analysis using ion exchange HPLC coupled with electrochemical detection |
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| Authors: | Murray D.B. Hayashida Y. Nishimura K. |
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| Affiliation: | (1) Biochemical Engineering Laboratory, National Institute for Bioscience and Human Technology, Tsukuba Ibaraki, 305, Japan;(2) Kumamoto Industrial Research Institute, Kumamoto, 368, Japan |
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| Abstract: | A method has been developed to detect trehalose in yeast extracts down to 10mM. A crude yeast extract was prepared by rapidly heating filtered cells to 95°C. Trehalose was separated using an ion exchange HPLC connected to an electrochemical detector. |
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