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A scalable membrane gradostat reactor for enzyme production using Phanerochaete chrysosporium |
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| Authors: | Govender S Jacobs E P Leukes W D Pillay V L |
| Affiliation: | (1) Department of Biochemistry, South Africa;(2) UNESCO Associated Centre for Macromolecules and Materials, Division of Polymer Science, Department of Chemistry, University of Stellenbosch, Private Bag X1, Matieland, 7602, South Africa;(3) Department of Chemical Engineering, Durban Institute of Technology, Durban, 4001, South Africa;(4) Department of Biochemistry & Microbiology, Rhodes University, P.O. Box 94, Grahamstown, 6140, South Africa |
| Abstract: | This is the first demonstration of process scale-up of a membrane gradostat reactor for continuous enzyme production using Phanerochaete chrysosporium ME446. The fungus was immobilised by reverse filtration on to externally unskinned, ultrafiltration capillary membranes and then nutrient gradients were induced across the biofilm. A 10-fold scale-up from a single capillary bioreactor to a 2.4 l multi-capillary unit resulted in a 7-fold increase in enzyme productivity with a peak at 209 U l–1 d–1. Subsequent scale effects on the spore distribution, continuous manganese peroxidase production profile and biofilm development are discussed. |
| Keywords: | gradostat immobilised spores membrane bioreactor Phanerochaete chrysosporium scale-up |
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