Distribution of peptidase activity in teleost and rat tissues |
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Authors: | N?Agirregoitia R?Laiz-Carriòn A?Varona M?P?Martín?del?Rio J?M?Mancera Email author" target="_blank">J?IrazustaEmail author |
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Institution: | (1) Department of Physiology, Faculty of Medicine and Dentistry, University of the Basque Country, P. O. Box 699, 48080 Bizkaia, Bilbao, Spain;(2) Department of Biology, Faculty of Sea and Environmental Sciences, University of Cádiz, , Spain |
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Abstract: | Peptides play important roles in cell regulation and signaling in many tissues. The actions of peptides are regulated by peptidases.
Although the activity of these enzymes has been thoroughly characterized in mammals, little is known about their presence
or function in fish. In the present study, we compared the activity of several peptidases in selected tissues (pituitary gland,
different brain areas, kidney and gills) of the gilthead sea bream and rainbow trout with that found in similar rat tissues
(lungs studied in place of gills). Soluble puromycin-sensitive aminopeptidase showed the highest values in the pituitary gland
of the sea bream, whereas the membrane-bound form was found to be more active in the trout kidney. Very high levels of activity
of aminopeptidase N were detected in trout and sea bream plasma. In contrast, the highest levels of activity of aminopeptidase
B were found in rat tissues, with the exception of the gills of the trout. Aminopeptidase N levels tended to be higher in
sea bream tissues with respect to those of trout. In contrast, the level of activity of aminopeptidase B was found to be consistently
much higher in trout tissues than in those of the sea bream. Prolyl endopeptidase activity was principally detected in the
pituitary gland and in the brain areas of teleosts. These differences between species could be related to different mechanisms
of osmoregulation in saltwater- and in freshwater-adapted fish. |
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Keywords: | Aminopeptidase Endopeptidase Gilthead sea bream Rainbow trout Sprague-Dawley rat |
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