Mouse cytosolic acetyl-CoA hydrolase,a novel candidate for a key enzyme involved in fat metabolism: cDNA cloning,sequencing and functional expression |
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Authors: | Suematsu Naoya Okamoto Kazuki Isohashi Fumihide |
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Institution: | Department of Biochemistry, St Marianna University School of Medicine, Kanagawa, Japan. |
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Abstract: | A cytosolic acetyl-CoA hydrolase (CACH) cDNA has been isolated from mouse liver cDNA library and sequenced. Recombinant expression of the cDNA in insect cells resulted in overproduction of active acetyl-CoA hydrolyzing enzyme protein. The mouse CACH cDNA encoded a 556-amino-acid sequence that was 93.5% identical to rat CACH, suggesting a conserved role for this enzyme in the mammalian liver. Database searching shows no homology to other known proteins, but reveals homological cDNA sequences showing two single-nucleotide polymorphisms (SNPs) in the CACH coding region. The discovery of mouse CACH cDNA is an important step towards genetic studies on the functional analysis of this enzyme by gene-knockout and transgenic approaches. |
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