2. Department of Physiology, Indian Institute of Experimental Medicine, 4, Raja S.C. Mullick Road, Calcutta-32 India;1. CSIR India
Abstract:
The cell suspension of Leishmania donovani incorporates 14CO2 resulting in the formation of 14C]-succinic acid under anaerobic conditions. The results showed that the 14C]-succinate formation from 1-14C]-glucose is much greater than that from 6-14C]-glucose. 14C-pyruvate] takes part in the production of succinic acid under anaerobic conditions without decarboxylation. The anaerobic formation of succinate appears to involve the production of malate, which is then converted to succinate via the reduction of fumarate by the reversal of the tricarboxylic acid cycle. Evidence indicated that the active species in this carboxylation reaction was CO2 although HCO3 was active to some extent.