Clonal fingerprinting in the genus <Emphasis Type="Italic">Populus</Emphasis> L. by nuclear microsatellite loci regarding differences between sections,species and hybrids |
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Authors: | Heike Liesebach Volker Schneck Elke Ewald |
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Institution: | 1.Institute of Forest Genetics,Johann Heinrich von Thünen-Institut, Federal Research Institute for Rural Areas, Forestry and Fisheries,Waldsieversdorf,Germany |
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Abstract: | Reliable methods for clone identification are desired to characterise and distinguish breeding products within the genus Populus L. (Salicaceae). Ten nuclear microsatellite loci (PMGC14, PMGC456, PMGC2163, PTR2, PTR7, WPMS05, WPMS09, WPMS14, WPMS15 and
WPMS20) were applied on a clone collection with several species and hybrids belonging to the sections Tacamahaca (balsam poplars), Aigeiros (black poplars, cottonwoods) and Populus (white poplars and aspens) and intersectional hybrids between black and balsam poplars. The members of the different sections
and species do not always share their allelic ladders. Some shifts of one or two nucleotides in allele length were observed
for several loci. This could be explained by nucleotide sequence differences in the flanking regions of loci in diverse taxonomic
groups. Such shifts of allelic ladders result in irregular patterns in hybrid genotypes. The set of loci should have a sufficient
amount of variation for a differentiation between clones, even if they are full siblings originating from crossing experiments. |
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