Immunological detection of proteolytically activated epidermal-type transglutaminase (TGase 3) using cleavage-site-specific antibody |
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| Authors: | Hitomi Kiyotaka Ikeda Naoki Maki Masatoshi |
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| Institution: | Department of Applied Molecular Biological Sciences, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Japan. hitomi@agr.nagoya-u.ac.jp |
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| Abstract: | Transglutaminase 3 (TGase 3), involved in the cross-linking of structural proteins in the epidermis, is activated by limited proteolysis of zymogen into two fragments during keratinocyte differentiation. Using recombinant TGase 3, the N-terminus sequence of the proteolyzed fragment was analyzed. Antibody against the synthetic peptide corresponding to the cleavage site specifically detected the fragment in the mouse forestomach extract. |
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