Localization of amplified CAD genes on rearranged chromosomes of Chinese hamster cells |
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| Authors: | Laura Ottaggio Cinzia Agnese Stefania Bonatti Pasqua Cavolina Aldo Di Leonardo Mariangela Miele Angelo Abbondandolo |
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| Institution: | (1) Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy;(2) Dipartimento di Biologia Cellulare e dello Sviluppo dell' Università di Palermo, Genova, Italy;(3) Instituto di Mutagenesi e Differenziamento CNR, Pisa, Italy;(4) Cattedra di Genetica, Università di Genova, Italy |
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| Abstract: | Chinese hamster cell lines carrying an amplified CAD region were selected from V79,B7 cells by their resistance to N-phosphonacetyl-L-aspartate (PALA). In one of the selected cell lines, SP PALA
inf1
supR
L, an acrocentric chromosome with abnormally elongated q arms was identified as a marker for the PALA-resistant phenotype. The marker chromosome carried a homogeneously staining region close to a telomeric nucleolar organizer region. In the same region, localization of amplified CAD sequences was demonstrated by in situ hybridization. The marker chromosome was found to undergo extensive rearrangements. In particular, dicentric chromosomes, occurring with an unusually high incidence, were found to originate from end-fusion of two homologous marker chromosomes.Abbreviations ATCase
Aspartate Transcarbamylase
- CAD
Carbamyl-phosphate synthetase-Aspartate transcarbamylase-Dihydroorotase
- MTX
Methotrexate
- NOR
Nucleolar Organizer Region
- PALA
N-phosphonacetyl-L-Aspartate |
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| Keywords: | CAD gene chromosome rearrangements gene amplification methotrexate-resistance PALA-resistance |
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