Localization of calcium in an annelid visceral muscle by pyroantimonate deposition and by X-ray microprobe analysis |
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Authors: | FI Alohan H Huddart |
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Institution: | Department of Biological Sciences, University of Lancaster, Lancaster LA1 4YQ, England |
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Abstract: | The loci of calcium distribution in Nereis pharangeal visceral muscle have been examined by cytochemical precipitation using potassium pyroantimonate. In Na-, Ca- and Mg-free media, pyroantimonate incubation was used to pinpoint loci of intracellularly bound calcium. This method also revealed heavy deposition on the inner face of the plasma membrane, in the sarcoplasmic reticulum and nucleus. X-ray microprobe analysis of the precipitate confirmed the presence of calcium and antimony peaks. It is concluded that the plasma membrane may constitute a major calcium pool for the activation of contraction in this muscle. |
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Keywords: | Correspondence and reprint requests to: Dr H Huddart Department of Biological Sciences University of Lancaster Lancaster L1A 4YQ England |
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