Quantitative phosphoproteomic analysis reveals reciprocal activation of receptor tyrosine kinases between cancer epithelial cells and stromal fibroblasts |
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Authors: | Xinyan Wu,Muhammad Saddiq Zahari,Santosh Renuse,Nandini A. Sahasrabuddhe,Min-Sik Kim,Mary Jo Fackler,Martha Stampfer,Edward Gabrielson,Saraswati Sukumar,Akhilesh Pandey |
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Affiliation: | 1.Department of Biological Chemistry,Johns Hopkins University,Baltimore,USA;2.McKusick-Nathans Institute of Genetic Medicine,Johns Hopkins University,Baltimore,USA;3.Institute of Bioinformatics, International Technology Park,Bangalore,India;4.Manipal Academy of Higher Education,Manipal,India;5.Department of Oncology,Johns Hopkins University School of Medicine,Baltimore,USA;6.Department of Pathology,Johns Hopkins University School of Medicine,Baltimore,USA;7.Division of Biological Systems and Engineering,Lawrence Berkeley National Laboratory,Berkeley,USA;8.Johns Hopkins University,Baltimore,USA |
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Abstract: | BackgroundCancer-associated fibroblasts (CAFs) are one of the most important components of tumor stroma and play a key role in modulating tumor growth. However, a mechanistic understanding of how CAFs communicate with tumor cells to promote their proliferation and invasion is far from complete. A major reason for this is that most current techniques and model systems do not capture the complexity of signal transduction that occurs between CAFs and tumor cells.MethodsIn this study, we employed a stable isotope labeling with amino acids in cell culture (SILAC) strategy to label invasive breast cancer cells, MDA-MB-231, and breast cancer patient-derived CAF cells. We used an antibody-based phosphotyrosine peptide enrichment method coupled to LC–MS/MS to catalog and quantify tyrosine phosphorylation-mediated signal transduction events induced by the bidirectional communication between patient-derived CAFs and tumor cells.ResultsWe discovered that distinct signaling events were activated in CAFs and in tumor epithelial cells during the crosstalk between these two cell types. We identified reciprocal activation of a number of receptor tyrosine kinases including EGFR, FGFR1 and EPHA2 induced by this bidirectional communication.ConclusionsOur study not only provides insights into the mechanisms of the interaction between CAFs and tumor cells, but the model system described here could be used as a prototype for analysis of intercellular communication in many different tumor microenvironments. |
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