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In vivo veritas: electron cryotomography of cells
Affiliation:1. College of Mechanical & Electrical Engineering, Hohai University, Changzhou 213022, China;2. Key Laboratory of Ministry of Education for Geomechanics and Embankment Engineering, Hohai University, Nanjing 210098, China;1. Department of Civil and Environmental Engineering, Yonsei University, Seoul 120-749, Republic of Korea;2. Department of Civil and Environmental Engineering, Western University, London, ON, Canada N6A 5B9;1. Department of Radiation Oncology, University of Texas Southwestern Medical Center, Dallas, TX;2. Department of Radiation Oncology, Rutgers Cancer Institute of New Jersey, New Brunswick, NJ;1. Nuclear Engineering Program, Mechanical and Aerospace Engineering, The Ohio State University Columbus, OH 43210, USA;2. Department of Chemistry, University of North Texas, Denton, TX 76203, USA;3. Elemental Measurement Science, Chemical Sciences Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA
Abstract:Automated electron cryotomography enables the study of organelles or whole cells embedded in vitrified ice in a close-to-life state and with a resolution of a few nanometers. This technology will provide new vistas of the supramolecular architecture inside cells that orchestrates higher cellular functions.
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