Immunodiagnosis of Parietaria mottle virus in Tomato Crops Using a Polyclonal Antiserum against its Coat Protein Expressed in a Bacterial System |
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| Authors: | Frederic,Aparicio ,José ,Aramburu ,Salvador,Soler ,Luis,Galipienso ,Fernando,Nuez ,Vicente,Pallá s Carmelo,Ló pez |
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| Affiliation: | Authors' addresses: Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia (UPV-CSIC), Avenida de los Naranjos s/n, 46022 Valencia, Spain;;Institut de Recerca i Tecnología Agroalimentaries (IRTA), Ctra. de Cabrils s/n, 08348 Cabrils, Barcelona, Spain;;Instituto de Conservación y Mejora de la Agrodiversidad Valenciana, Universidad Politécnica de Valencia (COMAV-UPV), Avenida de los Naranjos, s/n, 46022 Valencia, Spain (correspondence to C. López. E-mail: ) |
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| Abstract: | Recombinant DNA technology was used to raise a polyclonal antiserum against the coat protein (CP) of Parietaria mottle virus (PMoV). The CP gene was expressed in Escherichia coli as a fusion to a 6xHis tag and purified by affinity chromatography. Recombinant purified protein was used as antigen to raise a polyclonal antiserum. This polyclonal antiserum consistently detected PMoV specifically infected tomato plants from different commercial tomato crops by indirect enzyme-linked immunosorbent assay (I-ELISA) and direct tissue-printing immunoassay (DTBIA). |
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| Keywords: | Parietaria mottle virus recombinant CP tomato polyclonal antiserum indirect enzyme-linked immunosorbent assay direct tissue-printing immunoassay |
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