B．animalis V9对腹泻动物的保护性作用及其机制研究

目的研究益生菌B.animalis V9对腹泻动物的保护作用及其对腹泻致病菌的拮抗效果。方法采用自制的腹泻SPF鼠，喂服B．animalis V9，观察并检测腹泻致病菌，并在体外试验益生菌B．animalisV9对腹泻致病菌的拮抗作用。结果SPF鼠致泻动物模型喂食B．animalisV9，剂量为1．0×10^8CFU/（只·d），3d后腹泻治愈率为90％，死亡率为9％，对照组自然恢复率为16％，死亡率为41％，其余仍有不同程度的腹泻现象。B．animalis V9在体外对志贺痢疾杆菌、沙门菌、铜绿假单胞菌和大肠埃希菌有不同程度的拮抗作用，共同培养60h后均未检测到上述4种致病菌。结论B．animalisV9可以有效的对腹泻小鼠进行治疗，其机制在于B．animalisV9对致泻菌株具有一定程度的拮抗作用。

Study on the protective effect and mechanism of B.animalis V9 on mice with diarrhea

Objective To investigate the protection of B. animalis V9 to animals with diarrhea and its antagonistic effect to pathogenic bacteria inducing diarrhea. Method SPF white mice with diarrhea were fed with B. animalis V9 at a dose of 1.0 × 10^8 CFU/d. Pathogenic bacteria were observed and detected. The antagonistic effect of B. animalis V9 to pathogenic bacteria in vitro was investigated. Result In test group, the cure rate was 90% and the death rate was 9% after 3 days, wihle in control group, the natural recovery rate was 16% and the death rate was 41%. B. animalis V9 showed antagonistic effect to Shigella dysenteriae, Salmonella, Pseudomonas Aeruginosa, Escherichia coli and the four pathogenic bacteria were not detected in the co-culture after 60 hours in vitro. Conclusion B. animalis V9 can cure diarrhea in white mice effectively due to its antagonistic effect to pathogenic bacteria inducing diarrhea.