Purification and crystallization of Lactobacillus casei folylpolyglutamate synthetase expressed in Escherichia coli. |
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| Authors: | V Cody J R Luft W Pangborn J Toy A L Bognar |
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| Institution: | Molecular Biophysics Department, Medical Foundation of Buffalo, NY 14203. |
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| Abstract: | Folylpolyglutamate synthetase (FPGS) from Lactobacillus casei has been crystallized with polyethylene glycol and acetate buffer at pH 5.0. The enzyme was obtained from Escherichia coli strain SF4 harboring the L. casei FPGS chromosomal gene on a pEMBL vector (pGT3-8.1). Crystals of the enzyme were obtained which diffract to 2.6 A resolution. The crystals are monoclinic, space group P2(1), with unit cell dimensions of a = 54.07 A, b = 45.83 A, c = 84.37 A and beta = 107.92 degrees. A unit cell contains one molecule of the 43,000 Da enzyme per asymmetric unit. A complete X-ray data set on the native crystals has been collected. |
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