Somatic embryogenesis and plant regeneration from embryogenic callus of soybean,Glycine max L. |
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Authors: | T D Ghazi H V Cheema M W Nabors |
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Institution: | (1) Tissue Culture for Crops Project, Department of Botany, Colorado State University, 80523 Fort Collins, CO, USA;(2) Department of Botany, Punjab University, 160 014 Chandigarh, India |
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Abstract: | During induction of somatic embryogenesis from immature embryos of soybean, a smooth-shiny and a rough callus were obtained. The smooth-shiny type developed from callus derived from cotyledonary tissue and cultured on media containing 10 mg/l 2,4-D. The rough type was derived from immature embryos, cotyledons, hypocotyls and hypocotyl segments from germinated seedlings on a medium containing various growth regulators. Plants were obtained from the smooth-shiny type but rough callus did not differentiate into plants. The conditions for formation of both callus types and regeneration of mature soybean plants from the smooth-shiny type were defined.Abbreviations 2,4-D
2,4-dichlorophenoxyacetic acid
- IAA
indoleacetic acid
- IBA
indolebutyric acid
- ABA
abscicic acid
- GA3
gibberellic acid
- BA
benzyladenine
- B5
Gamborg et al.
- LS
Linsmaier & Skoog
- MS
Murashige & Skoog
- P
proline |
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