Plasma Membrane-Associated Glycohydrolases Activation by Extracellular Acidification due to Proton Exchangers |
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Authors: | Massimo Aureli Nicoletta Loberto Rosaria Bassi Anita Ferraretto Silvia Perego Patrizia Lanteri Vanna Chigorno Sandro Sonnino Alessandro Prinetti |
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Institution: | 1.Dipartimento di Chimica, Biochimica e Biotecnologie per la Medicina,Università degli Studi di Milano,Segrate,Italy |
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Abstract: | In this paper, we show that the pH optimum for the plasma membrane (PM)-associated activity of four glycohydrolases (conduritol
B epoxide sensitive β-glucosidase, β-glucosidase GBA2, β-hexosaminidase and β-galactosidase) measured on intact cells is acidic.
Moreover, we show that drugs able to modify the efflux of protons across the PM, thus locally affecting the extracellular
proton concentration close to the PM, are able to modulate the activities of these enzymes. These data strongly suggest that
pH-dependent modulation of PM-associated glycohydrolases activities could be an effective way to locally modulate the cell
surface glycoconjugate composition. |
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