| Ad-IL-24对SGC-7901胃癌细胞生长抑制的体外实验 |
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| 引用本文： | 包婉蓉,缪竞诚,盛伟华,单云波,李正祎,汪小华,井莹莹,韩亚丽,杨吉成. Ad-IL-24对SGC-7901胃癌细胞生长抑制的体外实验[J]. 生物工程学报, 2009, 25(10): 1586-1592 |
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| 作者姓名： | 包婉蓉 缪竞诚 盛伟华 单云波 李正祎 汪小华 井莹莹 韩亚丽 杨吉成 |
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| 作者单位： | 苏州大学医学部基础医学与生命科学学院细胞与分子生物学教研室,苏州,215123 |
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| 基金项目： | 江苏省高校自然科学基础研究项目(No.08KJB310011)资助~~ |
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| 摘 要： | 旨在研究携带人IL-24基因的腺病毒表达载体(Ad-IL-24)对SGC-7901人胃癌细胞的生长抑制作用并分析其分子机制。以不同MOI(感染复数)的Ad空载体腺病毒感染SGC-7901人胃癌细胞,筛选出最佳感染剂量;Ad-IL-24以最佳感染剂量感染SGC-7901胃癌细胞,RT-PCR法和Western blotting法检测腺病毒介导的IL-24基因在SGC-7901胃癌细胞中的转录;MTT法检测Ad-IL-24对SGC-7901胃癌细胞的生长抑制作用,流式细胞仪检测其诱导SGC-7901人胃癌细胞凋亡和细胞周期改变的效应,Hoechst33258染色荧光显微镜检测其诱导胃癌细胞凋亡的核形态改变;RT-PCR半定量法进一步检测SGC-7901胃癌细胞中凋亡相关基因的转录。结果显示,100MOI为感染SGC-7901胃癌细胞的腺病毒最佳感染剂量;Ad-IL-24能成功介导IL-24基因在SGC-7901胃癌细胞中转录性表达;Ad-IL-24感染SGC-7901胃癌细胞后,能明显抑制胃癌细胞生长和诱导凋亡;Ad-IL-24能显著上调SGC-7901胃癌细胞中bax、caspase-3和p53的表达和下调bc...
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| 关 键 词： | 腺病毒 人白介素-24 胃癌 肿瘤基因治疗 抗肿瘤效应 |
| 收稿时间： | 2009-06-02 |
Adenovirus mediated IL-24 gene expression suppresses gastric cancer cell growth in vitro |
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| Wanrong Bao,Jingcheng Miao,Weihua Sheng,Yunbo Shan,Zhengyi Li,Xiaohua Wang,Yingying Jing,Yali Han and Jicheng Yang. Adenovirus mediated IL-24 gene expression suppresses gastric cancer cell growth in vitro[J]. Chinese journal of biotechnology, 2009, 25(10): 1586-1592 |
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| Authors: | Wanrong Bao Jingcheng Miao Weihua Sheng Yunbo Shan Zhengyi Li Xiaohua Wang Yingying Jing Yali Han Jicheng Yang |
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| Affiliation: | Department of Celluar and Molecular Biology, Medical School, Soochow University, Suzhou 215123, China,Department of Celluar and Molecular Biology, Medical School, Soochow University, Suzhou 215123, China,Department of Celluar and Molecular Biology, Medical School, Soochow University, Suzhou 215123, China,Department of Celluar and Molecular Biology, Medical School, Soochow University, Suzhou 215123, China,Department of Celluar and Molecular Biology, Medical School, Soochow University, Suzhou 215123, China,Department of Celluar and Molecular Biology, Medical School, Soochow University, Suzhou 215123, China,Department of Celluar and Molecular Biology, Medical School, Soochow University, Suzhou 215123, China,Department of Celluar and Molecular Biology, Medical School, Soochow University, Suzhou 215123, China and Department of Celluar and Molecular Biology, Medical School, Soochow University, Suzhou 215123, China |
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| Abstract: | |
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| Keywords: | adenovirus interleukin-24 gastric cancer cancer gene therapy anti-tumor effect |
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