| GDAF对原代培养脊髓神经元的影响 |
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| 引用本文： | 陈哲宇 路长林. GDAF对原代培养脊髓神经元的影响[J]. 实验生物学报, 2000, 33(3): 237-244 |
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| 作者姓名： | 陈哲宇 路长林 |
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| 摘 要： |
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| 关 键 词： | 原代培养 脊髓神经元 神经营养因子 GDNF |
Effects of GDNF on primary culture of spinal cord neurons] |
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| Z Y Chen,A J Huang,X Bao,C L Lu. Effects of GDNF on primary culture of spinal cord neurons][J]. Acta Biologiae Experimentalis Sinica, 2000, 33(3): 237-244 |
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| Authors: | Z Y Chen A J Huang X Bao C L Lu |
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| Affiliation: | Department of Neurobiology, Second Military Medical University, Shanghai 200433. |
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| Abstract: | The effect of GDNF on long-term cultured spinal cord neurons was studied. GDNF could promote spinal cord neurons survival after 7 d or 14 d culture by MTT assay. The effect of GDNF on growth cones, neuron soma magnitude, neurite length and spines formulation of spinal cord neurons in cell culture was observed by phase microscopy, Nissl stain and NSE immunocytochemistry stain. The results indicated that GDNF had significant trophic effects on long-term cultured spinal cord neurons. |
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