A test for endogenous interference in superoxide dismutase assays |
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Authors: | G E Eldred J R Hoffert |
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Affiliation: | Department of Physiology, Michigan State University, East Lansing, Michigan 48854 USA |
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Abstract: | Endogenous interfering substances can be detected by applying the techniques of parallel-line analysis of variance to the assay of superoxide dismutase (SOD) activities in crude tissue extracts. The technique also allows expression of specific activities in terms of units of activity as defined by commercially available standards as opposed to definition by the specific assay conditions utilized. This analysis may be broadly applied to many of the indirect SOD assays currently in use. In the current investigation, SOD was assayed by its ability to inhibit the rate of reduction of acetylated ferricytochrome c by superoxide anion () generated via the xanthine-xanthine oxidase reaction. The parallel-line analysis was effective in detecting the presence of interference by exogenous ascorbate in the reaction system, and by unidentified endogenous reactants within extracts of whole blood and ocular choroidal tissues of the rainbow trout, Salmo gairdneri. |
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Keywords: | Present address and reprint requests to: University of Missouri School of Medicine Department of Ophthalmology N309 University Medical Center Columbia Mo. 65201. |
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