| 转基因烟草生产霍乱毒素B亚单位的纯化 |
| |
| 引用本文： | 王新国,张艳红,刘传暄,肖成祖,张国华,方荣祥.转基因烟草生产霍乱毒素B亚单位的纯化[J].生物技术通讯,2000,11(2):103-106. |
| |
| 作者姓名： | 王新国 张艳红 刘传暄 肖成祖 张国华 方荣祥 |
| |
| 作者单位： | 1. 军事医学科学院生物工程研究所,北京,100071
2. 中国科学院微生物所植物生物技术实验室,北京,100080 |
| |
| 摘 要： | 提取转基因烟草叶片总蛋白，用经溴化氰活化的Ｓｅｐｈａｒｏｓｅ ４Ｂ偶联有抗ＣＴ ＩｇＧ色谱柱，得到了表达产物ＣＴＢ蛋白质。经ＰＡＧＥ、Ｗｅｓｔｅｒｎ ｂｌｏｔ、琼脂糖免疫扩散和免疫电泳等方法鉴定表明该蛋白与天然ＣＴＢ相同。
|
| 关 键 词： | 霍乱毒素B亚单位 纯化 转基因烟草 |
Purification of cholera toxin B subunit from transgenic tobacco |
| |
| Wang Xinguo,Zhang Yanhong,Liu Chuanxuan,Xiao Chengzu,Zhang Guohua,Fang Rongxiang.Purification of cholera toxin B subunit from transgenic tobacco[J].Letters in Biotechnology,2000,11(2):103-106. |
| |
| Authors: | Wang Xinguo Zhang Yanhong Liu Chuanxuan Xiao Chengzu Zhang Guohua Fang Rongxiang |
| |
| Abstract: | CTB was purified from transgenic tobacco by affinity chromatography, and analyzed by SDS-PAGE, Western blot, immunodiffusion and immunoelectrophoresis. The results indicated that plant-derived CTB protein was antigenically indistinguishable from native CTB protein, and that oligomeric CTB molecules were the dominant molecular species isolated from transgenic tobacco leaves. |
| |
| Keywords: | cholera toxin B subunit affinity chromatography immunogenicity |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
