Chromosomal localization of four isozyme loci by trisomic analysis in rice (Oryza sativa L.) |
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| Authors: | S. Ranjhan J. C. Glaszmann D. A. Ramirez G. S. Khush |
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| Affiliation: | (1) The International Rice Research Institute, P.O. Box 933, Manila, Philippines;(2) Centre de Coopération Internationale en Recherche Agronomique pour le Développement, BP 5035, F-34032 Montpellier Cedex, France;(3) University of the Philippines at Los Baños, Laguna, Philippines |
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| Abstract: | Summary Four genes coding for isozymes in rice (Oryza sativa L.), were located to respective chromosmes through trisomic analysis. Twelve primary trisomics in IR36 background were crossed with 2 lines having contrasting alleles at four loci. For each gene, all 12 disomic and trisomic F1 hybrids were screened for allele dosage effects. Either F2 or BC1 populations of all cross combinations were assessed for gene segregtion. Evidence from both sources indicated the following locations: Pgi-1 on chromosome 4, Sdh-1 on chromosome 6, Est-8 on chromosome 7 and Adh-1 on chromosome 11. The location of Sdh-1 was further confirmed through the production of triallelic heterozygotes with trisomic 6. |
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| Keywords: | Isozymes Chromosomal location Trisomic analysis Gene dosage effects |
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