Effect of growth substrate,method of fermentation,and nitrogen source on lignocellulose-degrading enzymes production by white-rot basidiomycetes |
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Authors: | Vladimir Elisashvili Eva Kachlishvili Michel Penninckx |
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Institution: | (1) Durmishidze Institute of Biochemistry and Biotechnology, 10 km Agmashenebeli kheivani, 0159 Tbilisi, Georgia;(2) Laboratoire de Physiologie et Ecologie Microbienne, Faculte des Sciences, Universite Libre de Bruxelles, c/o Institut Pasteur, 642 Rue Engeland, 1180 Brussels, Belgium |
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Abstract: | The exploration of seven physiologically different white rot fungi potential to produce cellulase, xylanase, laccase, and
manganese peroxidase (MnP) showed that the enzyme yield and their ratio in enzyme preparations significantly depends on the
fungus species, lignocellulosic growth substrate, and cultivation method. The fruit residues were appropriate growth substrates
for the production of hydrolytic enzymes and laccase. The highest endoglucanase (111 U ml−1) and xylanase (135 U ml−1) activities were revealed in submerged fermentation (SF) of banana peels by Pycnoporus coccineus. In the same cultivation conditions Cerrena maxima accumulated the highest level of laccase activity (7,620 U l−1). The lignified materials (wheat straw and tree leaves) appeared to be appropriate for the MnP secretion by majority basidiomycetes.
With few exceptions, SF favored to hydrolases and laccase production by fungi tested whereas SSF was appropriate for the MnP
accumulation. Thus, the Coriolopsis polyzona hydrolases activity increased more than threefold, while laccase yield increased 15-fold when tree leaves were undergone
to SF instead SSF. The supplementation of nitrogen to the control medium seemed to have a negative effect on all enzyme production
in SSF of wheat straw and tree leaves by Pleurotus ostreatus. In SF peptone and ammonium containing salts significantly increased C. polyzona and Trametes versicolor hydrolases and laccase yields. However, in most cases the supplementation of media with additional nitrogen lowered the fungi
specific enzyme activities. Especially strong repression of T. versicolor MnP production was revealed. |
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Keywords: | White-rot basidiomycetes Lignocellulose fermentation Cellulase Laccase Manganese peroxidase |
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