A Useful Cloning Vector for Bacillus subtilis |
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Authors: | B rge Diderichsen, Gitte Bak Poulsen,Steen T. J rgensen |
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Affiliation: | Børge Diderichsen, Gitte Bak Poulsen,Steen T. Jørgensen |
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Abstract: | We have constructed plasmid pDN1050 a new small cloning vector for Bacillus subtilis . pDN1050 harbors the origin of replication of Staphylococcus aureus plasmid pUB110 and the chloramphenicol resistance gene of S. aureus plasmid pC194. The plasmid is segregationally and structurally stable. Plasmid pDN1370, a low copy number mutant of pDN1050 was isolated and shown to harbor a mutation in the repA gene of the replication protein. |
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