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Generation of free radicals and initiation of radical reactions in nitrones - Fe2+ - phosphate buffer systems |
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| Authors: | S Tero-Kubota Y Ikegami T Kurokawa R Sasaki K Sugioka M Nakano |
| Affiliation: | Department of Pathology, University of Otago, Christchurch Clinical School of Medicine, Christchurch, New Zealand. |
| Abstract: | Three antigenic fractions were isolated from collagenase-solubilised human glomerular basement membrane utilising affinity columns containing purified human anti-glomerular basement membrane autoantibodies. The three fractions were able to inhibit the binding of sera containing anti-glomerular basement membrane autoantibodies when measured by solid phase radio-immunoassay. Amino acid and carbohydrate analyses revealed differences in the composition of the three fractions and were consistent with them being derived from the non-collagenous components of glomerular basement membrane. |
| Keywords: | BSA bovine serum albumin GBM glomerular basement membrane FCS foetal calf serum PBS phosphate buffered saline PVC polyvinyl chloride RIA radioimmunoassay SDS-PAGE sodium-dodecyl sulphate polyacrylamide gel electrophoresis |
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