Improvement of in vitro androgenesis in niger using amino acids and polyamines |
| |
Authors: | B P Hema H N Murthy |
| |
Institution: | 1. Plant Tissue Culture Laboratory, Department of Botany, Karnatak University, Dharwad, 580 003, India
|
| |
Abstract: | The effects of amino acids (arginine, aspargine, cysteine, glutamine, glycine and proline) and polyamines (putrescine and spermidine) on embryogenesis and plant regeneration from cultured anthers of Guizotia abyssinica (L. f.) Cass. cv. Ootacamund was studied. Supplementation of amino acids (0.5–5.0 mM) to the induction medium individually and in combination, improved embryo yield. B5 medium supplemented with 2 mM proline, 10 µM 2,4-dichlorophenoxyacetic acid, 2 µM kinetin and 0.2 M sucrose induced highest number of embryos (63 per 60 anthers cultured). Addition of polyamines (5–200 µM) to the same medium also enhanced the rate of embryogenesis. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|