Effects of oxygen volumetric mass transfer coefficient and pH on lipase production by Staphylococcus warneri EX17 |
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Authors: | Giandra Volpato Rafael C Rodrigues Júlio Xandro Heck Marco Antônio Záchia Ayub |
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Institution: | 1. Department of Chemical Engineering, Federal University of Rio Grande do Sul State, Rua Professor Luiz Englert, s/n, ZC 90040-040, Porto Alegre, RS, Brazil 2. Food Science and Technology Institute, Federal University of Rio Grande do Sul State, Av. Bento Gon?lves, 9500, P.O. Box 15090, ZC 91501-970, Porto Alegre, RS, Brazil 3. Technical School, Federal University of Rio Grande do Sul State, Rua Ramiro Barcelos, 2777, ZC 90035-007, Porto Alegre, RS, Brazil
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Abstract: | The principal objectives of this study were to evaluate the kinetics of lipase production by Staphylococcus warneri EX17 under different oxygen volumetric mass transfer coefficients (kLa) and pH conditions in submerged bioreactors, using glycerol (a biodiesel by-product) as a carbon source. Cultivations were
conducted at different kLa (26, 38, 50, and 83 h−1) and pH values (6.0, 7.0, and 8.0). The optimal kLa and pH were 38 h−1 and 7.0, respectively. Under these conditions, the maximal cell production obtained was 8.0 g/L, and the volumetric and specific
lipase production reached high levels of activity, approximately 800 U/L and 150 U/g cell, respectively, after 12 h of cultivation.
This result was approximately five times higher than that obtained in the shake flask cultures. The relationship between cell
growth and lipase production was found to be associated with growth by the Luedeking-Piret model. |
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Keywords: | lipase Staphylococcus warneri bioreactor oxygen volumetric mass transfer coefficient (kLa) |
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