A complex role of Amycolatopsis mediterranei GlnR in nitrogen metabolism and related antibiotics production |
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Authors: | Hao Yu Yufeng Yao Yang Liu Ruishen Jiao Weihong Jiang Guo-Ping Zhao |
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Affiliation: | (1) Laboratory of Molecular Microbiology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai, 200032, People’s Republic of China;(2) State Key Laboratory of Genetic Engineering, Department of Microbiology, School of Life Science, Fudan University, 220 Handan Road, Shanghai, 200433, People’s Republic of China;(3) Laboratory of Disease and Health Genomics, Chinese National Human Genome Center at Shanghai, Bi-Bo Road, Shanghai, 201203, People’s Republic of China;(4) Present address: 13 Natural Resources BLDG, East Lansing, MI 48823, USA;(5) Present address: Division of Pediatric Infectious Diseases, School of Medicine, Johns Hopkins University, Baltimore, MD 21287, USA;(6) Present address: Biomedical Engineering, and Anatomy & Cell Biology, Indiana University – Purdue University Indianapolis, Indiana, USA |
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Abstract: | Amycolatopsis, genus of a rare actinomycete, produces many clinically important antibiotics, such as rifamycin and vancomycin. Although GlnR of Amycolatopsis mediterranei is a direct activator of the glnA gene expression, the production of GlnR does not linearly correlate with the expression of glnA under different nitrogen conditions. Moreover, A. mediterranei GlnR apparently inhibits rifamycin biosynthesis in the absence of nitrate but is indispensable for the nitrate-stimulating effect for its production, which leads to the hyper-production of rifamycin. When glnR of A. mediterranei was introduced into its phylogenetically related organism, Streptomyces coelicolor, we found that GlnR widely participated in the host strain’s secondary metabolism, resemblance to the phenotypes of a unique S. coelicolor glnR mutant, FS2. In contrast, absence or increment in copy number of the native S. coelicolor glnR did not result in a detectable pleiotrophic effect. We thus suggest that GlnR is a global regulator with a dual functional impact upon nitrogen metabolism and related antibiotics production. |
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Keywords: | Amycolatopsis mediterranei GlnR Nitrate stimulating effect |
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