Regulation of the murine TR2/HVEM gene expression by IRF |
| |
Authors: | Sul Ok-Ju Lee Hyeon-Woo Kim Woon-Ki Choi Jin-Hee Kwak Jung-Sook Choi Youn-Jung Latour Anne M Koller Beverly H Jeong Choon-Soo Kwon Byoung S |
| |
Affiliation: | Department of Biological Science and the Immunomodulation Research Center, University of Ulsan, San29 Mugeo-Dong, Nam-Gu, Ulsan 680-749, Republic of Korea. |
| |
Abstract: | TR2 (TNFR-related 2, HVEM, or TNFRSF-14), a member of the TNFR family, is involved in a number of immune responses. While TR2 is expressed on the surface of T cells during the resting state, little is known regarding how expression of the TR2 gene is regulated. To understand the mechanisms regulating the expression of TR2 in T cells, we analyzed the 5' flanking region of TR2. We identified an important region for the activity of the TR2 promoter using site directed mutagenesis. Using EMSA analysis, we found that IRF-2 was bound to the promoter region of the TR2 gene during the resting state of EL-4 T cells. Transfection of IRF-2 expression plasmid and of dominant negative IRF-2 mutant further confirmed our results. Together, these data demonstrate that IRF-2 is involved in the regulation of TR2 expression in EL-4 T cells. |
| |
Keywords: | |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|