| Abstract: | Although most duplex DNAs are not immunogenic some synthetic DNAs such as polyd(Tm5C)].polyd(GA)] are weakly immunogenic allowing the production of monoclonal antibodies. The specificity of one of these antibodies, Jel 172, was investigated in detail by a competitive solid-phase radioimmune assay. Jel 172 bound well to polyd(TC)].polyd(GA)] but not to other duplex DNAs such as polyd(TTC)].polyd(GAA)] and polyd(TCC)].polyd(GGA)]. The binding to polyd(Br5UC)].polyd(GA)] was enhanced while that to polyd(TC)].polyd(IA)] was decreased compared to polyd(TC)].polyD(GA)]. Thus, not only is the antibody very specific for a sequence of duplex DNA but it also appears to recognize functional groups in both grooves of the helix. |
