| 番木瓜抗病突变体阻碍环斑病毒体内运转 |
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| 引用本文： | 周国辉,LI Hua-ping,李华平,张曙光,范怀忠. 番木瓜抗病突变体阻碍环斑病毒体内运转[J]. 中国病毒学, 2001, 16(1): 55-58 |
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| 作者姓名： | 周国辉 LI Hua-ping 李华平 张曙光 范怀忠 |
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| 作者单位： | 1. 华南农业大学植物病毒研究室,
2. Plant virology laboratory ,South China Agricultural U niversity |
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| 基金项目： | 广东省重点科技项目（99M04202G）资助。 |
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| 摘 要： | 应用RT-PCR一步法检测了PRSVYs株系在感病番木瓜及其抗病突变体植株体内的运转动态，结果表明在感病植株中，接种后48hr接种叶的未接种部位可检出病毒，第4天部分接种叶柄可检出病毒，第6天植株各部位均能检出病毒；而在抗病植株中，接种后可以而且仅能在接种部位检出病毒；因而认为抗病突变体能够阻碍病毒从接种部位运出及（或）向未接种部位运入。
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| 关 键 词： | 番木瓜环斑病毒；反转录PCR；病毒运转；抗病突变体 |
| 文章编号： | 1003-5125(2001)01-0055-04 |
| 修稿时间： | 1999-12-30 |
Block of Viral Movement inPapaya Mutant Plants Resistant to Papaya Ringspot Virus |
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| ZHoU Guohui,LI Hua—ping ZHANG Shu—guang FAN Hwai—zhong. Block of Viral Movement inPapaya Mutant Plants Resistant to Papaya Ringspot Virus[J]. Virologica Sinica, 2001, 16(1): 55-58 |
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| Authors: | ZHoU Guohui LI Hua—ping ZHANG Shu—guang FAN Hwai—zhong |
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| Affiliation: | 1.Plant virology laboratory，South China Agricultural University，Guangzhou 510642，China |
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| Abstract: | One-step RT-PCR was used to detect papaya ringspot virus (PRSV ) moveme nt in susceptible and resistant (mutant) papaya (Carica papaya L.) plants. The t ime of post-inoculation at which PRSV was first detected in susceptible plants w as 48 hours in the other area of inoculated leaves out of inoculated sites , 4 d ays in the petioles of inoculated leaf and 6 days in all parts of plant. Compara tively, in resistant plants, the inoculated sites were the only area at which PR SV can be detected any time. Therefore, it was proposed that the mutated resista nce to PRSV protects the virus from moving out of inoculated sites or/and into non-inoculated areas. |
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| Keywords: | Papaya ring spot virus~RT-PCR Viral movement Resistant mutant |
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