| 广西绞股蓝的组织培养和快速繁殖 |
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| 引用本文： | 刘世彪,李馨芸,龙华,蔡松.广西绞股蓝的组织培养和快速繁殖[J].氨基酸和生物资源,2012,34(1):29-32. |
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| 作者姓名： | 刘世彪 李馨芸 龙华 蔡松 |
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| 作者单位： | 1. 吉首大学生物资源与环境科学学院,湖南吉首,416000;2. 湖南湘泉制药有限公司,湖南吉首,416000 |
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| 基金项目： | 湖南省自然科学基金重点资助项目（09JJ3051）; 湖南省高校产业化培育项目（10CY015） |
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| 摘 要： | 广西绞股蓝是极具开发潜力的绞股蓝属植物。以其茎尖和叶片为外植体进行的组织培养试验表明,茎尖为最适的外植体,愈伤组织诱导和不定芽增殖的最适培养基为MS+6-BA 1.0mg.L-1+NAA 0.02mg.L-1,在此培养基中,茎尖和叶片的愈伤组织诱导率平均达95%,丛芽增殖系数平均达14。广西绞股蓝最适的生根培养基为MS+NAA 0.20mg.L-1,生根率92.50%。炼苗后组培苗的移栽成活率达95.5%。
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| 关 键 词： | 广西绞股蓝 组织培养 快速繁殖 |
Tissue Culture and Rapid Propagation of Gynostemma guangxiense |
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| LIU Shibiao
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LI Xinyun
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LONG Hua
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CAI Song.Tissue Culture and Rapid Propagation of Gynostemma guangxiense[J].Amino Acids & Biotic Resources,2012,34(1):29-32. |
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| Authors: | LIU Shibiao LI Xinyun LONG Hua CAI Song |
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| Institution: | 1.College of Biology and Environmental Science,Jishou University,Jishou 416000,Hunan,China; 2.Xiangquan Pharmaceutical Company Limited of Hunan,Jishou 416000,Hunan,China) |
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| Abstract: | Gynostemma guangxiense is a potential species that could be developed.The tissue culture experiment with shoot tips and blades as explants showed that shoot tips were the best explants.The culture medium of MS＋6-BA 1.0 mg·L-1＋NAA 0.02 mg·L-1 was appropriate for inducing callus and multiplying lush buds.The induction frequency of callus was 95% and the proliferation coefficient of adventitious bud reached 14 for the shoot tips and blades cultured on this medium.MS＋NAA 0.20 mg·L-1 had a better effect on inducing roots with 92.5% of growing root percentage.95.5% survival percentage of transplanting was obtained after hardening-seedling. |
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| Keywords: | Gynostemma guangxiense tissue culture rapid propagation |
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