Identification of superoxide dismutase isoenzymes in tobacco pollen |
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| Authors: | Jing Wang Xuequn Liu Guanghui Yu |
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| Affiliation: | 1. Key Laboratory for Biotechnology of the State Ethnic Affairs Commission, College of Life Science, South-Central University for Nationalities, Wuhan, 430074, China
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| Abstract: | We investigated the possible existence of superoxide dismutase (SOD; EC 1.15.1.1) isoenzymes in the pollen of Nicotiana tabacum (Petit Havana SR-1 cultivar). To detect SOD activity, crude extracts from tobacco pollen were subjected to native polyacrylamide gel electrophoresis followed by staining with nitroblue tetra-zolium (NBT). The presence of six SOD isoenzymes was detected in tobacco pollen. Treatment with SOD inhibitors indicated the presence of one manganese SOD (Mn SOD), five copper-zinc SOD (Cu/Zn SOD) isoenzymes, and the absence of iron SOD (Fe SOD). |
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| Keywords: | tobacco pollen reactive oxygen species superoxide dismutase fertilization |
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