Intrachromosomal recombination between attP regions as a tool to remove selectable marker genes from tobacco transgenes |
| |
Authors: | Zubko E Scutt C Meyer P |
| |
Institution: | Leeds Institute for Plant Biotechnology and Agriculture (LIBA), Faculty of Biological Sciences, The University of Leeds, Leeds LS2 9JT, United Kingdom. |
| |
Abstract: | Recombinant genes conferring resistance to antibiotics or herbicides are widely used as selectable markers in plant transformation. Once transgenic material has been selected, the marker gene is dispensable. We report a novel strategy to remove undesirable parts of a transgene after integration into the tobacco genome. This approach is based on the transfer of a vector containing a NPTII gene flanked by two 352 bp attachment P (attP) regions of bacteriophage lambda, and the identification of somatic tissue with deletion events following intrachromosomal recombination between the attP regions. This system was used to delete a 5.9 kb region from a recombinant vector that had been inserted into two different genomic regions. As the attP system does not require the expression of helper proteins to induce deletion events, or a genetic segregation step to remove recombinase genes, it should provide a useful tool to remove undesirable transgene regions, especially in vegetatively propagated species. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|