Keratinolytic proteases of <Emphasis Type="Italic">Bacillus</Emphasis> species isolated from the Amazon basin showing remarkable de-hairing activity |
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| Authors: | Janice Luhering Giongo Françoise S Lucas Franciani Casarin Philipp Heeb Adriano Brandelli |
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| Institution: | 1.Laboratório de Bioquímica e Microbiologia Aplicada, ICTA,Universidade Federal do Rio Grande do Sul,Porto Alegre,Brasil;2.Department of Ecology and Evolution,University of Lausanne,Lausanne,Switzerland;3.Cereve, UPVM-Faculté des Sciences et Technologies,Créteil Cedex,France;4.Laboratoire Evolution et Diversité Biologique,Université Paul Sabatier III,Toulouse, Cedex 9,France |
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| Abstract: | Three keratinolytic Bacillus spp. isolated from the Brazilian Amazon basin were characterized. The strains P6, P7 and P11 were identified based on morphological
and biochemical characteristics and 16S rDNA sequences. P6, P7 and P11 sequences shared more than 99% similarity with B. subtilis, B. amyloliquefaciens and B. velesensis. The keratinases produced by these bacteria were active on azokeratin and degradation of feather barbules was observed. The
enzymes were inhibited by the serine protease inhibitor PMSF, and showed maximum activity at pH 9.0. Proteins like albumin,
casein and gelatin were hydrolysed by these keratinases. Depilatory studies on bovine pelts revealed that all three strains
were efficient in promoting de-hairing. Microscopic analysis showed that the epidermis was completely removed and the absence
of hair in follicles was observed. |
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| Keywords: | Bacillus Protease Keratinase Feather Leather Depilatory activity |
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