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Formation of monoclonal antibody against a major ginseng component, ginsenoside Rg1 and its characterization. Monoclonal antibody for a ginseng saponin |
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| Authors: | Noriko Fukuda Hiroyuki Tanaka Yukihiro Shoyama |
| Affiliation: | (1) Department of Pharmacognosy, Graduate school of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higahsi-ku, Fukuoka, 812,-8582, Japan;(2) Department of Pharmacognosy, Graduate school of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higahsi-ku, Fukuoka, 812,-8582, Japan |
| Abstract: | The ratio of hapten and bovine serum albumin in an antigenconjugate was determined by matrix-assisted laserdesorption/ionization time-of-flight mass spectrometry. Ahybridoma secreting monoclonal antibody against ginsenosideRg1 was produced by fusing sprenocytes immunized with aginsenoside Rg1-bovine serum albumin conjugate withHAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. A very smallcross-reaction appeared with ginsenoside Re. The full measuringrange of the assay extends from 0.3  g ml-1 to 10 g ml-1 ofginsenoside Rg1. |
| Keywords: | ELISA ginsenoside Rg1 mass spectrometry monoclonal antibody qualitative analysis |
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