Thin-layer chromatographic determination of indolic tryptophan metabolites in human urine using Sep-Pak C18 extraction |
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Authors: | Domenica Tonelli Enrico Gattavecchia Massimo Gandolfi |
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Affiliation: | Istituto di Scienze Chimiche, Università di Bologna, Via San Donato 15, 40127 Bologna Italy |
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Abstract: | Tryptophan and some of its indole metabolites were separated by thin-layer chromatography, stained with the Van Urk—Salkowski reagent, and quantitated by scanning densitometry. The application of this technique for the detection of the indoles in urine samples, employing Sep-Pak C18 cartridges for extraction, was demonstrated. The proposed method is simple and accurate. The detection limits were 2 μg/ml 5-hydroxytryptophan, 1.75 μg/ml 5-hydroxyindolyl-3-acetic acid, 1.5 μg/ml tryptophan, 0.8 μg/ml indolyl-3-acetic acid, 0.9 μg/ml indolyl-3-butyric acid, 1.75 μg/ml serotonin, and 1.25 μg/ml tryptamine. |
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