Microcalorimetric evaluation of the effects of methotrexate and 6-thioguanine on sensitive T-lymphoma cells and on a methotrexate-resistant subline |
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| Authors: | Jordi Bermudez Per Bäckman Arne Schön |
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| Institution: | (1) Facultat d’Odontologia, Universitat de Barcelona, Feixa Llarga, E-08907 L’Hospitalet, Barcelona, Spain;(2) Division of Thermochemistry, Chemical Center, University of Lund, PO Box 124, S-221 00 Lund, Sweden |
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| Abstract: | Isothermal microcalorimetry was used in order to continuously monitor and quantitatively assess the action of two antineoplastic
drugs, methotrexate (MTX) and 6-thioguanine (6-TG), on a human T-lymphoma cell line, CCRF-CEM. The results with MTX were compared
with data from experiments with a MTX-resistant subline, CEM/MTX. The slope of the power-time curve after drug injection relative
to that obtained during unperturbed growth, was used to construct dose-response curves. The normal cell line was characterized
by aD
50 value (i.e., the dose producing half the maximal response) of 0.05 μM for MTX and 0.38 μM for 6-TG. For the MTX-resistant subline theD
50 value was 8 μM of MTX. Comparisons of the continuous power-time curves showed the inhibitory effect of 6-TG to be faster than that of MTX. |
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| Keywords: | Microcalorimetry on T-lymphoma cells 6-thioguanine methotrexate methotrexate resistant cells dose-response curves |
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