A 36-kDa mitochondrial protein is responsible for cyanide-resistant respiration in Hansenula anomala |
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| Authors: | N Minagawa S Sakajo T Komiyama A Yoshimoto |
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| Institution: | Department of Biochemistry, Niigata College of Pharmacy, Japan. |
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| Abstract: | Antimycin A-dependent induction of cyanide-resistant respiration in Hansenula anomala was reversibly blocked by carbonylcyanide-m-chlorophenylhydrazone (CCCP). When the cells were pulse-labeled with 35S]methionine in the presence of both antimycin A and CCCP, the radioactivity was incorporated into a 39 kDa mitochondrial protein. Upon removal of CCCP, this protein was processed into a 36 kDa form. The increase in the 36 kDa protein completely paralleled that in cyanide-resistant respiration activity, suggesting that the 39 kDa protein is the precursor of the 36 kDa protein, which is responsible for cyanide-resistant respiration. |
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