Recombinant plasmids with genes for the biosynthesis of alkaline phosphatase of Escherichia coli |
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Authors: | Werner Boidol, Marianne Simonis, Michael Tö pert Gerhard Siewert |
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Affiliation: | (1) Research Laboratories of Schering AG Berlin (West) and Bergkamen, Müllerstr. 170/178, D-1000 Berlin 65 |
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Abstract: | Summary Restriction maps of several recombinant plasmids representing a section of the E. coli K12 chromosome 35,000 bp in size with the genes phoA, proC and phoB were prepared. The orientation of phoA and the exact position of its N-terminal end on this map were determined by identifying a subfragment which carried the phoA promoter and by determining the nucleotide sequence of a 160 bp portion of this subfragment comprising the codons for the N-terminal end of pre-alkaline phosphatase. From this DNA sequence the leader sequence of alkaline phosphatase which consists of 21 amino acids was derived. |
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