声波刺激对猕猴桃愈伤组织ATP含量的影响

采用单因子实验设计,分别先固定作为交变应力的声波的频率或强度这两个参数中的一个,而改变另一个指标的值,用声波刺激木本植物中华猕猴桃(Actinidiachinensis)茎段的愈伤组织,并测定对比声波处理前后其ATP含量及变化情况。实验结果表明,声波对猕猴桃愈伤组织ATP的含量有着比较明显的增强或抑制的双重效应,适度的声场刺激将有利于提高植物的能量代谢水平,其中,最适的声波频率为1000Hz,最适声强为100dB左右 。

Effects of Sound Stimulation on ATP Content of Actinidia chinensis Callus

Using callus cultured from stalks of Actinidia chinensis,this study dealed with the effects of sound stimulation on plant cell energy metabolism i.e.content of ATP.The results indicated that there occurs bidirectional effects of ATP content on sound field stimulation.The sound intensity of about 100 dB and sound frequency of approximate 1000 Hz are optimal external stresses for energy metabolism of A.chinensis.The experiment showed that moderate sound field would be advantageous to energy metabolism and development of woody plant.The mechanisms of ATP content effects of sound stimulation on A.chinensis callus was comprehensively discussed in the light of cytobiology and molecular biology.While the essential mechanisms of biological effects of environmental stresses remains further research.;