Iron binding proteins and influx of irom across the duodenal brush border: Evidence for specific lactotransferrin receptors in the human intestine |
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Authors: | Timothy M Cox Jöel Mazurier Geneviève Spik Jean Montreul Timothy J Peters |
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Institution: | 1. Cell Biology Unit, Department of Medicine, Royal Postgraduate Medical School, London U.K.;2. Laboratoire de Chimie Biologique de l''Université des Sciences et Techniques de Lille I et Laboratoire Associé au CNRS No. 217 B.P. No. 36, F-59650 Villeneuve d''Ascq France |
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Abstract: | The ability of a range of homologous transferrin-like proteins to donate iron to pieces of human duodenal mucosa, was examined with an in vitro incubation technque. In contrast to serum transferrin and ovotransferrin, only lactotransferrin was able to yield its iron to intestinal tissue, but in an autologous system this protein was unable to donate iron to human reticulocyte preparations. Studies with 125I-labelled lactotransferrin and lactotransferrin dual-labelled with 59Fe and 125I, indicated that the intact protein is excluded from entry into the enterocytes. The experiments suggest that iron may be transported across the brush border after delivery to specific protein binding sites at the cell surface. |
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Keywords: | Iron absorption Enterocyte Lactotransferrin Receptor Serum transferrin (Duodenum) Hepes |
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