Molecular mechanisms of olfactory reception IV. Some biochemical characteristics of the camphor receptor from rat olfactory epithelium |
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Authors: | EE Fesenko VI Novoselov LD Krapivinskaya |
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Institution: | Institute of Biological Physics, Academy of Sciences of the USSR, Pushchino, Moscow Region U.S.S.R. |
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Abstract: | Some parameters of the receptor element from the rat olfactory epithelium are evaluated; it is characterized by a high affinity for camphor (KD = 1.5 · 10?9 M). Triton X-100 has no marked effect on the binding of 3H]camphor. Neither RNAase nor phospholipase C affected 3H]camphor-binding activity. Pronase and trypsin abolished 3H]camphor binding activity by 65 and 40%, respectively. Sulfhydryl reagents decrease the binding of 3H]camphor by a factor of 5–8. The isoelectric point of the receptor solubilized with Triton X-100 is 4.8, as determined by isoelectric focusing. The molecular weight of the receptor as determined by gel electrophoresis is about 120 000. It is proposed that the camphor receptor is a membrane protein containing sulfhydryl groups and playing a key role in olfactory reception. |
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Keywords: | Olfaction Receptor Camphor (Rat olfactory mucosa) |
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