| 多药抗药基因Mdrl探针的克隆及初步应用 |
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| 引用本文： | 胡美茹,汲言山,舒翠玲,陈立军,沈倍奋.多药抗药基因Mdrl探针的克隆及初步应用[J].生物化学与生物物理进展,1995,22(4):334-337. |
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| 作者姓名： | 胡美茹 汲言山 舒翠玲 陈立军 沈倍奋 |
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| 作者单位： | 北京军事医学科学院基础医学研究所;北京军事医学科学院基础医学研究所;北京军事医学科学院基础医学研究所;北京军事医学科学院基础医学研究所;北京军事医学科学院基础医学研究所 |
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| 摘 要： | 多药抗药基因Mdrl的表达水平与细胞的耐药性直接相关,检测Mdrl的表达水平可预测化疗的效果以及预后,用分子原位杂交的方法可检测单个细胞中Mdrl的表达水平.用PCR扩增方法获得了一段特异的DNA片段,并将其克隆到pUC18载体中,经DNA序列分析证明与文献报道一致,此探针可用于临床标本的分子杂交检测.
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| 关 键 词： | Mdrl 多药抗药（MDR） 基因克隆 |
| 收稿时间： | 8/9/1994 12:00:00 AM |
| 修稿时间： | 1994/1/24 0:00:00 |
Cloning of Mdrl Gene Probe |
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| Hu Meiru,Ji Yanshan,Shu Cuiling,Chen Lijun and Shen Beifen.Cloning of Mdrl Gene Probe[J].Progress In Biochemistry and Biophysics,1995,22(4):334-337. |
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| Authors: | Hu Meiru Ji Yanshan Shu Cuiling Chen Lijun and Shen Beifen |
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| Institution: | Institute of Basic Medical Science, Academy of Military Medical Sciences;Institute of Basic Medical Science, Academy of Military Medical Sciences;Institute of Basic Medical Science, Academy of Military Medical Sciences;Institute of Basic Medical Science, Academy of Military Medical Sciences;Institute of Basic Medical Science, Academy of Military Medical Sciences |
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| Abstract: | Mdrl gene contributes to multidrug resistance.Because of the level of Mdrl gene expression is correlated with the response of chemotherapy,it is a valuable method to measure the expression level of individual patients. The cDNA cloning of the human Mdrl gene has made it possible to measure levels of Mdrl RNA with hybridization in human cancer cells. A special part of Mdrl cDNA was obtained by use of RT-pCR and inserting it into pUC18 vector.The DNA sequencing result is identical with the sequence of Mdrl cDNA reported. This probe can be used to detect the level of Mdrl gene expression in clinical specimen. The measurements can be useful in the design of chemotherapeutic protocols for certain tumors. |
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| Keywords: | Mdrl MDR gene cloning |
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