Architectural Features of theSalmonella typhimuriumFlagellar Motor Switch Revealed by Disrupted C-Rings |
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Authors: | Shahid Khan Rongbao Zhao Thomas S Reese |
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Institution: | aDepartment of Physiology &; Biophysics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York, 10461;bLaboratory of Neurobiology, NINDS, NIH, Bethesda, Maryland, 20892 |
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Abstract: | The three-dimensional surface topology of rapid-frozenSalmonella typhimuriumflagellar hook basal body complexes was studied by stereo-examination of thin-film metal replicas. The complexes contained the extended cytoplasmic structure, composed of the switch complex proteins; FliG, FliM, and FliN. Distinct nanometer-scale element arrays, separated by grooves, defined the outer surface of the cytoplasmic (C-) ring. The number of array elements was comparable to previously determined FliG and FliM copy numbers in the basal body. In addition to basal body complexes lacking C-rings, complexes containing incomplete C-rings were identified. The incomplete C-rings had lost segments of the proximal array. Basal bodies with the distal C-ring array alone were not found. These findings are compatible with the spatial organization of the flagellar switch suggested by previous biochemical data. |
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